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e coli ec1000 strain96  (Addgene inc)


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    Structured Review

    Addgene inc e coli ec1000 strain96
    E Coli Ec1000 Strain96, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+ec1000+strain96/EC1000+(Bacterial+strain+%2371852)/pm39163861-1090-3-7
    Average 93 stars, based on 6 article reviews
    e coli ec1000 strain96 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
    Article Snippet: For plasmid construction, E. coli EC1000 strain96 (Addgene #71852), a kanamycin resistant strain carrying a single copy of the repA gene in the glgB gene, was used as the cloning host for all pORI28-based plasmids, and E. coli MC1061 strain (Molecular Cloning Laboratories #MC1061) was used as the cloning host for all pTRK892-based plasmids. .. Cell 187, 1–18.e1–e16, September 19, 2024 e9 ll OPEN ACCESS Please cite this article in press as: Zhu et al., Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.029 Article Plasmid transformations methods for strain generation in L. gasseri ATCC 33323 Competent cells of E. coli EC1000 strain were prepared using the Mix & Go! .. E. coli Transformation Kit (Zymo Research #T3001) following the manufacturer’s protocol.

    Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
    Article Snippet: For Gibson cloning, Gibson Assembly Master Mix (NEB #E2611) was used for all Gibson assembly reactions following the manufacturer’s protocol. .. For plasmid construction, E. coli EC1000 strain96 (Addgene #71852), a kanamycin resistant strain carrying a single copy of the repA gene in the glgB gene, was used as the cloning host for all pORI28-based plasmids, and E. coli MC1061 strain (Molecular Cloning Laboratories #MC1061) was used as the cloning host for all pTRK892-based plasmids. .. Cell 187, 1–18.e1–e16, September 19, 2024 e9 ll OPEN ACCESS Please cite this article in press as: Zhu et al., Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.029 Article Plasmid transformations methods for strain generation in L. gasseri ATCC 33323 Competent cells of E. coli EC1000 strain were prepared using the Mix & Go!

    Cloning:

    Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
    Article Snippet: For Gibson cloning, Gibson Assembly Master Mix (NEB #E2611) was used for all Gibson assembly reactions following the manufacturer’s protocol. .. For plasmid construction, E. coli EC1000 strain96 (Addgene #71852), a kanamycin resistant strain carrying a single copy of the repA gene in the glgB gene, was used as the cloning host for all pORI28-based plasmids, and E. coli MC1061 strain (Molecular Cloning Laboratories #MC1061) was used as the cloning host for all pTRK892-based plasmids. .. Cell 187, 1–18.e1–e16, September 19, 2024 e9 ll OPEN ACCESS Please cite this article in press as: Zhu et al., Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.029 Article Plasmid transformations methods for strain generation in L. gasseri ATCC 33323 Competent cells of E. coli EC1000 strain were prepared using the Mix & Go!

    Molecular Cloning:

    Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
    Article Snippet: For Gibson cloning, Gibson Assembly Master Mix (NEB #E2611) was used for all Gibson assembly reactions following the manufacturer’s protocol. .. For plasmid construction, E. coli EC1000 strain96 (Addgene #71852), a kanamycin resistant strain carrying a single copy of the repA gene in the glgB gene, was used as the cloning host for all pORI28-based plasmids, and E. coli MC1061 strain (Molecular Cloning Laboratories #MC1061) was used as the cloning host for all pTRK892-based plasmids. .. Cell 187, 1–18.e1–e16, September 19, 2024 e9 ll OPEN ACCESS Please cite this article in press as: Zhu et al., Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment, Cell (2024), https://doi.org/10.1016/j.cell.2024.07.029 Article Plasmid transformations methods for strain generation in L. gasseri ATCC 33323 Competent cells of E. coli EC1000 strain were prepared using the Mix & Go!



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